Determination of p-Anisidine in workplace air by HPLC / 中华劳动卫生职业病杂志

Objective@#To establish an HPLC method for determining p-Anisidine in workplace air.@*Methods@#An C18 capillary column was used with the detection wavelength of 240 nm, the methanol solution (v/v=62:38) as mobile phase, the flow rate was 0.9 ml/min, and the injection volume was 20 μl.@*Results@#The linear range (μg/ml) for p-Anisidine was 0~100; correlation coefficient was 0.999 7; the detection limit was 0.15 μg/ml; the average precision of RSD was 4.4%. The sampling efficiency was more than 95% using silicone tube as sampling filter. Sample can be stored at room temperature for more than 7 d.@*Conclusion@#the method appered to be accurate, simple, sensitive and reliable, also have a high sampling efficiency, and can be usd to determine p-anisidine in workplace air simultaneously.

Relationship between occupational stressors and serum levels of thyroid hormones in policemen / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To explore the relationship of occupational stressors with the serum levels of triiodothyronine (T3), thyroxine (T4), and thyroid stimulating hormone (TSH).</p><p><b>METHODS</b>Using convenience sampling and cluster sampling methods, 225 policemen from a local police station in China were enrolled as subjects. Questionnaires were used to investigate demographic features and occupational stressors in those subjects. The serum levels of T3, T4, and TSH were measured by radioimmunoassay. The SPSS 13.0 software was used to perform t test or analysis of variance, partial correlation analysis, and multivariate non-conditional logistic regression analysis.</p><p><b>RESULTS</b>Reward was positively correlated with the level of T3(P<0.05). Daily tension was positively correlated with the level of T4(P<0.05). Psychological demand, effort, and daily tension were negatively correlated with the level of TSH (all P<0.05). The quality of sleep was positively correlated with the level of TSH (P<0.05). The logistic regression analysis revealed that the risk of increase in T3 level in the group with a high score for daily tension was 3.19-fold higher than that in the group with a low score, while the risk of increase in T3 level in the group with a high score for negative emotion was 1.32-fold higher than that in the group with a low score. The risk of increase in TSH level in the group with a high score for negative emotion was 0.43-fold that in the group with a low score.</p><p><b>CONCLUSION</b>The occupational stressors are correlated with the serum levels of thyroid hormones. Occupational stress can result in an increase in T3 level and a decrease in TSH level. However, occupational stress has no effect on T4 level.</p>

The relationship of occupational stress and the level of some hormone metabolites in urine / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To explore the possibility for 17-OHCS, 17-KS, VMA and HVA in urine as indicators of occupational stress identification and evaluation.</p><p><b>METHODS</b>225 policies were investigated by using convenience sampling and cluster sampling methods, occupational stressors, strains, buffer factors and individual factors were measured by questionnaires. The urine 17-OHCS, 17-KS, VMA and HVA level was tested by ELISA and high performance liquid chromatography method respectively.</p><p><b>RESULTS</b>The urine 17-OHCS level of the groups with high coworker support and job control scores was lower than that of the groups with low score group (P < 0.05), the urine 17-OHCS level of the group with high negative affectivity score was higher than that of the group with low score group (P < 0.05). The urine 17-KS level of the group with high job security score was lower than that of the counterparts (P < 0.05). The urine VMA level of the group with high coworker support score was lower than that of the counterparts (P < 0.05). The urine HVA level of the group with high coping strategy score was lower than that of counterpart (P < 0.05). Logistic regression analysis revealed that the job control and respect were the protective factors of urine 17-OHCS level increase (OR = 0.353 and 0.352 respectively), but over-commitment, self-esteem and daily hassles were the risk factors of urine 17-OHCS with level increase (OR = 3.391, 2.439, and 2.584 respectively). The risk of urine 17-KS level increase for the groups high job control and job security scores were respectively 0.387, 0.356 times than that of the counterparts, the risk of urine VMA level increase for the group with high negative affectivity score was 2.643 times than that of the counterparts.</p><p><b>CONCLUSION</b>The urine 17-OHCS, 17-KS and VMA level were potential indicators for occupational stress evaluation.</p>

Determination of four biogenic amine metabolites in urine by high-performance liquid chromatography / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To establish a method for simultaneously determining vanilmandelic acid (VMA), 5-hydroxyindoleacetic (5-HIAA), 3, 4-dihydroxyphenylacetic acid (DOPAC), and homovanillic acid (HVA) in urine by high-performance liquid chromatography (HPLC).</p><p><b>METHODS</b>After being filtered with a 0.45 µm membrane syringe filter, the urinary samples were injected directly into the HPLC system using a C18 chromatographic column and a fluorescence detector. The excitation and emission wavelengths were chose as 280 nm and 315 nm, respectively, and the urinary samples were carried with a mobile phase of methanol-0.1 mol/L phosphate buffered solution (V/V = 20:80) at a flow rate of 1.0 ml/min and an injection volume of 20 µl.</p><p><b>RESULTS</b>Using the method reported here, the correlation coefficients of VMA, 5-HIAA, DOPAC, and HVA were 0.9999, 0.9998, 0.9997, 0.9999, respectively, over linear ranges of 0-2.5, 0-2.0, 0-2.0, and 0-2.5 µg/ml, the minimum detectable concentrations were 0.006, 0.008, 0.012, and 0.0082 µg/ml, the average precisions were 4.2%, 3.7%, 4.9%, and 3.6%, and the recovery rates were 91%∼102%, 93%∼101%, 94%∼101%, and 89%∼ 102%.</p><p><b>CONCLUSION</b>This determination method is simple, efficient, accurate, and sensitive for the simultaneous detection of VMA, 5-HIAA, DOPAC, and HVA in urine.</p>

Study on the correlation of the biochemical indexes in flouride workers / 中华劳动卫生职业病杂志

<p><b>OBJECTIVE</b>To explore the correlation among some biochemical indexes in the fluoride workers.</p><p><b>METHODS</b>The activities of superoxide dismutase(SOD), glutathione peroxidase (GSH-Px), catalase (CAT), alkaline phosphatase (AKP) and the level of calcitonin (CT), parathyroid hormone (PTH), IgG, IgA, IgM, Cu2+, Zn2+, Ca2+, Mg2+ and Se2+, F- in serum and in urine were measured in fifty male fluoride workers and fifty controls.</p><p><b>RESULTS</b>The levels of F-, CT, PTH, AKP and GSH-Px in serum and F- in urine in exposed group were significantly different from that in control group. Correlation analysis indicated that F- in urine and CAT(r = 0.3133, P < 0.05), CT and PTH(r = 0.5173, P < 0.01), Se2+ and CAT(r = 0.4354, P < 0.05) were positively correlated. There were significantly negative correlation between F- in serum and GSH-Px (r = -0.5202, P < 0.01) and positive correlation among Cu2+, Zn2+, Ca2+ and Mg2+ in serum.</p><p><b>CONCLUSION</b>(1) Excess of fluoride may affect secretion of calcium adjusting hormone (CT, PTH); (2) Changes of AKP and GSH-Px may be regarded as health monitoring indexes; (3) The correlation of biochemical indexes plays an important role in studying the mechanism and the early prevention and treatment of industrial fluorosis.</p>